学习经历
2004.09-2009.07 中国科学院物理研究所 博士 软物质物理与生物物理
2000.09-2004.07 山东大学物理学院 学士 物理学基地班
工作经历与任职情况
2020.9- ：教授，博士生导师 金沙8888js官方
2015.11-2020.8：研究员，博士生导师 金沙8888js官方，武汉大学高等研究院
2010.03-2015.10：研究科学家 新加坡-MIT联合科研中心生物系统与微观力学实验室
2009.07-2010.03：专利审查员 国家知识产权局
荣誉与奖励
湖北省高层次人才青年项目入选者（2018）
国家高层次人才青年项目入选者（2022）
主要研究领域及兴趣
使用单分子磁镊技术和单分子FRET技术，揭示RNA 、DNA、蛋白质等生物大分子的动态结构及相互作用。使用结构生物信息学方法，揭示生物大分子的结构动态和功能机制。
DNA甲基化、单分子检测技术、核小体和染色质的动态调控是我们目前的研究重点。
承担课程（本科生、研究生）
主讲课程：《生物物理学》、《高等生物物理学》、《单分子生物学》、《生物化学实验》
主持课题项目：
张兴华 教授
国家自然科学基金（2017、2018、2020、2024）
国家高层次人才计划青年项目基金（2022）
杨亚军 助理研究员
国家自然科学基金（2022）
张晨 助理研究员
国家自然科学基金（2023、2024）
代表性成果(#为共同第一作者，*为通讯作者)
通讯作者论文（标*）：
1. Ya-Jun Yang, Hang Fu, Xiao-Lu Li, Hong-Yu Yang, Er-Chi Zhou, Cheng-Yu Xie, Shu-Wen Wu, Fan He*, Yan Zhang* and Xing-Hua Zhang*, “A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.”, Nucleic Acids Research, gkad601 (2023)
2. Fu-Jia Tian#, Chen Zhang #, Erchi Zhou#, Hai-Long Dong, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*，“Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding.”PNAS, 120 (20) e2218425120 (2023)
3. Xiao-Cong Zhao#, Hai-Long Dong#, Xiao-Lu Li, Hong-Yu Yang, Xue-Feng Chen, Liang Dai, Wen-Qiang Wu, Zhi-Jie Tan* and Xing-Hua Zhang*, “5-methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations.” Nucleic Acids Research, 50 (21): 12344–12354 (2022)
4. Chen Zhang#, Fujia Tian#, Ying Lu, Bing Yuan, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*, “Twist-diameter coupling drives DNA twist changes with salt and temperature.” Science Advances, 8(12), abn1384 (2022)
5. Xiao-Wei Qiang#, Chen Zhang#, Hai-Long Dong#, Fu-Jia Tian, Hang Fu, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*, Zhi-Jie Tan*, “Multivalent cations reverse the twist-stretch coupling of RNA.” Physical Review Letters, 126, 108103. (2022)
6. Xuejie Wang#, Yang Dong#, Xiaocong Zhao#, Jinbao Li, Jordan Lee, Zhenxin Yan, Shuangshuang Yang, Wenqiang Wu, Ximiao Hou, Guangxue Liu, Yueyue Zhang, Lun Song, Gang Cai, Qing Li, Grzegorz Ira, Xinghua Zhang*, Xuefeng Chen*, “Rtt105 promotes high-fidelity DNA replication and repair by regulating the single-stranded DNA binding factor RPA.” PNAS, 118 (25): e2106393118 (2021)
7. Ya-Jun Yang, Hai-Long Dong, Xiao-Wei Qiang, Hang Fu, Er-Chi Zhou, Chen Zhang, Lei Yin, Xue-Feng Chen, Fu-Chao Jia, Liang Dai, Zhi-Jie Tan, and Xing-Hua Zhang*. “Cytosine methylation enhances DNA condensation revealed by equilibrium measurements using magnetic tweezers”. Journal of the American Chemical Society. 142 (20): 9203–9209 (2020)
8. Hang Fu#, Chen Zhang#, Xiao-Wei Qiang#, Ya-Jun Yang, Zhi-Jie Tan* and Xing-Hua Zhang*. “Opposite effects of high-valent cations on the elasticities of DNA and RNA duplexes revealed by magnetic tweezers.” Physical Review Letters. 124(5): 058101 (2020)
9. Chen Zhang#, Hang Fu#, Yajun Yang#, Erchi Zhou, Zhijie Tan, Huijuan You, Xinghua Zhang*. “The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers”. Biophysical Journal. 116 (2): 196-204 (2019) [期刊年度最佳论文]
10. Ya-Jun Yang#, Lun Song, Xiao-Cong Zhao, Chen Zhang, Wen-Qiang Wu, Hui-Juan You, Hang Fu, Er-Chi Zhou, Xing-Hua Zhang*. “A Universal Assay for Making DNA, RNA, and RNA–DNA Hybrid Configurations for Single-Molecule Manipulation in Two or Three Steps without Ligation”. ACS synthetic biology. 8(7): 1663-1672 (2019)
11. Ju-Hui Liu #, Kun Xi #, Xi Zhang, Lei Bao, Xing-Hua Zhang*, Zhi-Jie Tan*. “Structural Flexibility of DNA-RNA Hybrid Duplex: Stretching and Twist-Stretch Coupling”. Biophysical Journal. 117 (1): 74-86 (2019)
12. Xiao-Cong Zhao #, Hang Fu, Lun Song, Ya-Jun Yang, Er-Chi Zhou, Guang-Xue Liu, Xue-Feng Chen, Zhuo Li, Wen-Qiang Wu, Xing-Hua Zhang*, “S‑DNA and RecA/RAD51-Mediated Strand Exchange in Vitro”. Biochemistry. 58 (15): 2009-2016 (2019) [IF 3.162]
第一作者论文：
1. Xinghua Zhang, Yuanyuan Qu, Hu Chen, Ioulia Rouzina, Shengli Zhang, Patrick S. Doyle*, and Jie Yan*, “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136(45): 16073-16080 (2014) [IF 15.419]
2. Xinghua Zhang, Hu Chen, Shimin Le, Ioulia Rouzina, Patrick S. Doyle*, and Jie Yan*, “Revealing the competition between peeled ssDNA, melting bubbles and S-DNA during DNA overstretching by single-molecule calorimetry”, PNAS, 110(10): 3865-3870 (2013) [IF 12.777]
3.Xinghua Zhang, Hu Chen, Hongixa Fu, Patrick S. Doyle*, and Jie Yan*, "Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements", PNAS, 109 (21): 8103-8108 (2012) [IF 12.777]
发明专利：
202310992413.1 《一种分子检测方法》
招生招聘
每年接收2-3名硕士生，2名博士生，1-2名博士后。
