教育经历/Education

1999/09-2004/06，武汉大学，博士/Wuhan University, PhD

1994/09-1998/07，武汉大学，学士/Wuhan University, Bachelor's Degree

工作经历/Professional Experience

2004/07-至今：金沙8888js官方/College of Life Sciences, Wuhan University

2014/02-2015/01：美国梅哈里医学院/Meharry Medical College, USA

1998/07-1999/08： 湖南省畜牧兽医研究所/Animal husbandry and veterinary institute of Hunan province

研究方向/Research Fields

1) 病毒感染及其防治/Viral infection, prevention and cure

2) 抗病原微生物多肽的药用基础/Medicinal basis of peptides against pathogen viruses and bacteria

3) 毒素多肽生物学/Toxic peptide biology

基金资助/Fund support

1) 国家优秀青年基金：蝎毒素遗传资源的保护、认识与利用研究

2) 湖北省杰出青年基金：靶向抗肿瘤蝎氯毒素的药用基础研究

3) 中央高校基本科研基金：蝎毒素多肽的药用基础研究

研究概述/Research Description

我过去以蝎为代表开展毒素多肽生物学研究，创建了蝎毒素多肽资源库，发现了蝎毒素多肽的分子多样性，首绘了蝎全基因组图谱，揭示了蝎毒素多肽分子多样性的产生机制，发掘了蝎毒素多肽的抗肿瘤、抗菌、抗病毒功能及作用机制，开拓了蝎毒素多肽研究和药用新领域。现在主要开展离子通道在病毒感染中作用以及在此基础上抗病毒多肽的筛选与设计研究。/ Before, I focused on the biology of toxin peptides (e.g. scorpion), involving in the library construction, diversity and its mechanism, pharmacological roles and their mechanisms of scorpion toxins. Today, I am interesting in the functions of ion channels in the infections of viruses, and then the discovery of antiviral peptides.

发表的代表性论文

1)Li X, Yang H, Han Y, Yin S, Shen B, Wu Y, Li W,Cao Z*. Tick peptides evoke itch by activating MrgprC11/X1 to sensitize downstream TRPV1 in pruriceptors.J Allergy Clin Immunol.2021 Jun;147(6):2236-2248. (IF=10.793)

2)Cheng Y, Sun F, Wang L, Gao M, Xie Y, Sun Y, Liu H, Yuan Y, Yi W, Huang Z, Yan H, Peng K, Wu Y,Cao Z*. Virus-induced p38 MAPK activation facilitates viral infection.Theranostics.2020 Oct 30;10(26):12223-12240.(IF=11.556)

3)Zeng Z, Zhang R, Hong W, Cheng Y, Wang H, Lang Y, Ji Z, Wu Y, Li W, Xie Y*,Cao Z*. Histidine-rich modification of a scorpion-derived peptide improves bioavailability and inhibitory activity against HSV-1.Theranostics. 2018, 8(1):199-211.(IF=11.556)

4)Cao Z^#, Yu Y^#, Wu Y^#, Hao P^#, Di Z^#, He Y^#, Chen Z, Yang W, Shen Z, He X, Sheng J, Xu X, Pan B, Feng J, Yang X, Hong W, Zhao W, Li Z, Huang K, Li T, Kong Y, Liu H, Jiang D, Zhang B, Hu J, Hu Y, Wang B, Dai J, Yuan B, Feng Y, Huang W, Xing X, Zhao G, Li X, Li Y, Li W. The genome of Mesobuthus martensii reveals a unique adaptation model of arthropods.Nat Commun. 2013,4:2602.(IF=14.919)

5) Hong W, Zhang R, Di Z, He Y, Zhao Z, Hu J, Wu Y, Li W,Cao Z*. Design of histidine-rich peptides with enhanced bioavailability and inhibitory activity against hepatitis C virus.Biomaterials. 2013,34(13):3511-3522.(IF=12.479)

6)Yu XF, Sun Z, Li M, Xiang Y, Wang QQ*, Tang F, Wu Y,Cao Z*, Li W*. Neurotoxin-conjugated upconversion nanoprobes for direct visualization of tumors under near-infrared irradiation.Biomaterials. 2010,31(33): 8724-8731.(IF=12.479)

7)Zeng Z, Han S, Hong W, Lang Y, Li F, Liu Y, Li Z, Wu Y, Li W, Zhang X*,Cao Z*. A tat-conjugated peptide nucleic acid Tat-PNA-DR inhibits hepatitis B virus replication in vitro and in vivo by targeting LTR direct repeats of HBV RNA.Mol Ther Nucleic Acids. 2016,5:e295.(IF=8.886)

8)Fan S, Sun Z, Jiang D, Dai C, Ma Y, Zhao Z, Liu H, Wu Y,Cao Z*, Li W*. BmKCT toxin inhibits glioma proliferation and tumor metastasis.Cancer Lett. 2010 ,291(2):158-166.(IF= 8.679)

9)Chen B, Li F, Zhu XK, Xie W, Hu X, Zan MH, Li X, Li QY, Guo SS, Zhao XZ, Jiang YA*,Cao Z*, Liu W*. Highly biocompatible and recyclable biomimetic nanoparticles for antibiotic-resistant bacteria infection.Biomater Sci.2021 Feb 9;9(3):826-834.(IF=6.843)

10)Sun F, Mu C, Kwok HF, Xu J, Wu Y, Liu W, Sabatier JM, Annweiler C, Li X*,Cao Z*, Xie Y*. Capivasertib restricts SARS-CoV-2 cellular entry: a potential clinical application for COVID-19.Int J Biol Sci.2021 Jun 11;17(9):2348-2355.(IF=6.58)

11)Xia Z, Wang L, Li S, Tang W, Sun F, Wu Y, Miao L*,Cao Z*. ML-SA1, a selective TRPML agonist, inhibits DENV2 and ZIKV by promoting lysosomal acidification and protease activity.Antiviral Res.2020 Oct;182:104922. (IF=5.97)

12)Hong W, Li T, Song Y, Zhang R, Zeng Z, Han S, Zhang X, Wu Y, Li W,Cao Z*. Inhibitory activity and mechanism of two scorpion venom peptides against herpes simplex virus type 1.Antiviral Res. 2014,102:1-10.(IF=5.97)

13)Liu G, Yang F, Li F, Li Z, Lang Y, Shen B, Wu Y, Li W, Harrison PL, Strong PN, Xie Y, Miller K*,Cao Z*. Therapeutic potential of a scorpion venom-derived antimicrobial peptide and its homologs against antibiotic-resistant gram-positive bacteria.Front Microbiol. 2018 May 29;9:1159.(IF=5.64)

14)Lang Y, Li F, Liu Q, Xia Z, Ji Z, Hu J, Cheng Y, Gao M, Sun F, Shen B, Xie C, Yi W, Wu Y, Yao J*,Cao Z*. The Kv1.3 ion channel acts as a host factor restricting viral entry.FASEB J. 2021 Feb;35(2):e20995.(IF=5.191)

15)Fan Z, Cao L, He Y, Hu J, Di Z, Wu Y, Li W*,Cao Z*. Ctriporin, a new anti-methicillin-resistant Staphylococcus aureus peptide from the venom of the scorpion Chaerilus tricostatus.Antimicrob Agents Chemother. 2011,55(11):5220-5229. (IF=5.191)

16)Zhao Z, Ma Y, Dai C, Zhao R, Li S, Wu Y,Cao Z*, Li W*. Imcroporin, a new cationic antimicrobial peptide from the venom of the scorpion Isometrus maculates.Antimicrob Agents Chemother. 2009,83:3472-3477. (IF=5.191)

17)Dai C, Ma Y, Zhao Z, Zhao R, Wang Q, Wu Y,Cao Z*, Li W*. Mucroporin, the first cationic host defence peptide from the venom of Lychas mucronatus.Antimicrob Agents Chemother. 2008,52(11): 3967-3972. (IF=5.191)

18)Li F, Lang Y, Ji Z, Xia Z, Han Y, Cheng Y, Liu G, Sun F, Zhao Y, Gao M, Chen Z, Wu Y, Li W,Cao Z*. A scorpion venom peptide Ev37 restricts viral late entry by alkalizing acidic organelles.J Biol Chem. 2019 Jan 4;294(1):182-194.(IF=5.157)

19)Meng L, Xie Z, Zhang Q, Li Y, Yang F, Chen Z, Li W,Cao Z*, Wu Y*. Scorpion potassium channel-blocking defensin highlights a functional link with neurotoxin.J Biol Chem. 2016,291(13):7097-106.(IF=5.157)

20)Hong W, Lang Y, Li T, Zeng Z, Song Y, Wu Y, Li W,Cao Z*. A p7 ion channel-derived peptide inhibits hepatitis C virus infection in vitro.J Biol Chem.2015,290(38):23254-23263.(IF=5.157)

21)Song Y, Gong K, Yan H, Hong W, Wang L, Wu Y, Li W*, Li W*,Cao Z*. Sj7170, a unique dual-functional peptide with a specific α-chymotrypsin inhibitory activity and a potent tumor activating effect from scorpion venom.J Biol Chem.2014,289(17):11667-11680.(IF=5.157)

22)Zhao Z, Hong W, Zeng Z, Wu Y, Hu K, Tian X, Li W*,Cao Z*. Mucroporin-M1 inhibits Hepatitis B virus replication by activating the mitogen-activated protein kinase (MAPK) Pathway and down-regulating HNF4αin vitroandin vivo.J Biol Chem.2012,287(36):30181-30190.(IF=5.157)

23)Sun F, Xia Z, Han Y, Gao M, Wang L, Wu Y, Sabatier JM, Miao L,Cao Z*. Topology, antiviral functional residues and mechnism of IFITM1.Viruses. 2020;12:295.(IF=5.048)

24)Li Z, Liu G, Meng L, Yu W, Xu X, Li W, Wu Y*,Cao Z*. K1K8: an Hp1404-derived antibacterial peptide.Appl Microbiol Biotechnol. 2016, 100(11):5069-5077.(IF=4.813)

25)Di Z, Yu Y, Wu Y, Hao P, He Y, Zhao H, Li Y, Zhao G, Li X*, Li W*,Cao Z*. Genome-wide analysis of homeobox genes from Mesobuthus martensii reveals Hox gene duplication in scorpions.Insect Biochem Mol Biol.2015,61:25-33.(IF=4.714)

26)Cheng Y, Sun F, Li S, Gao M, Wang L, Sarhan M, Abdel-Rahman MA, Li W, Kwok HF, Wu Y,Cao Z*. Inhibitory activity of a scorpion defensin BmKDfsin3 against hepatitis C virus.Antibiotics (Basel). 2020 Jan 17;9(1).(IF=4.639)

27)Zeng Z, Zhang Q, Hong W, Xie Y, Liu Y, Li W, Wu Y,Cao Z*. A scorpion defensin BmKDfsin4 inhibits hepatitis B virus replication in vitro.Toxins (Basel). 2016,8(5).(IF=4.546)

28)Cao Z^#, Di Z^#, Wu Y, Li W. Overview of scorpion species from China and their toxins.Toxins(Basel). 2014 Feb 26;6(3):796-815.(IF=4.546)

29)Annweiler C*,Cao Z*, Sabatier JM. Point of view: Should COVID-19 patients be supplemented with vitamin D?Maturitas. 2020 Oct;140:24-26. (IF=4.342)

30)Zhang C, Xie Z, Li X, Chen J, Feng J, Lang Y, Yang W, Li W, Chen Z, Yao J,Cao Z*, Wu Y*. Molecular basis for the toxin insensitivity of scorpion voltage-gated potassium channel MmKv1.Biochem J. 2016,473(9):1257-1266.(IF=3.857)

31)Zhao R, Ma Y, He Y, Di Z, Wu Y,Cao Z*, Li W*. Comparative venom gland transcriptome analysis of the scorpion Lychas mucronatus reveals intraspecific toxic gene diversity and new venomous components.BMC Genomics. 2010,11: 452.(IF=3.969)

32)Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y,Cao Z*, Li W*. Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.BMC Genomics. 2009,10: 290.(IF=3.969)

33)He Y, Zhao R, Di Z, Li Z, Xu X, Hong W, Wu Y, Zhao H, Li W,Cao Z*. Molecular diversity of Chaerilidae venom peptides reveals the dynamic evolution of scorpion venom components from Buthidae to non-Buthidae.J Proteomics. 2013,89:1-14.(IF=4.044)

34)Ma Y, He Y, Zhao R, Wu Y, Li W*,Cao Z*. Extreme diversity of scorpion venom peptides and proteins revealed by transcriptomic analysis: Implication for proteome evolution of scorpion venom arsenal.J Proteomics. 2012,75(5):1563-1576.(IF=4.044)

35)Ma Y, Zhao Y, Zhao R, Zhang W, He Y, Wu Y,Cao Z*, Guo L*, Li W*. Molecular diversity of toxic components from the scorpion Heterometrus petersii venom revealed by proteomic and transcriptome analysis.Proteomics. 2010,10(13):2471-2485.(IF=3.984)

36)Ji Z, Li F, Xia Z, Guo X, Gao M, Sun F, Cheng Y, Wu Y, Li W, Ali SA,Cao Z*. The scorpion venom peptide Smp76 inhibits viral infection by regulating type-I interferon response.Virol Sin. 2018 Dec;33(6):545-556.(IF=4.327)

37)Shen B, Song J, Zhao Y, Zhang Y, Liu G, Li X, Guo X, Li W,Cao Z*, Wu Y*. Triintsin, a human pathogenic fungus-derived defensin with broad-spectrum antimicrobial activity.Peptides. 2018 Sep;107:61-67.(IF=3.75)

38)Lang Y, Pi X, Di Z, Zhang Q, Wang H, Shen B, Li F, Liu G, Yu Y, Li X, Wu Y, Li W,Cao Z*. Molecular characterization and expression analysis of CSαβ defensin genes from the scorpion Mesobuthus martensii.Biosci Rep.2017 Dec 15;37(6).(IF=3.84)

39)Xu T, Fan Z, Li W, Dietel B, Wu Y, Beckmann MW, Wrosch JK, Buchfelder M, Eyupoglu IY,Cao Z*, Savaskan NE*. Identification of two novel chlorotoxin derivatives CA4 and CTX-23 with chemotherapeutic and anti-angiogenic potential.Sci Rep.2016 Feb 2;6:19799.(IF=4.379)

40)Li Z, Xu X, Meng L, Zhang Q, Cao L, Li W, Wu Y,Cao Z*. Hp1404, a new antimicrobial peptide from the scorpion Heterometrus petersii.PLoS One. 2014 May 14;9(5):e97539.(IF=3.24)

41)Chen Y, Cao L, Zhong M, Zhang Y, Han C, Li Q, Yang J, Zhou D, Shi W, He B, Liu F, Yu J, Sun Y, Cao Y, Li Y, Li W, Guo D,Cao Z*, Yan H*. Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7.PLoS One. 2012,7(4):e34947.(IF=3.24)

42)Cao L, Dai C, Li Z, Fan Z, Song Y, Wu Y,Cao Z*, Li W*. Antibacterial activity and mechanism of a scorpion venom Peptide derivative in vitro and in vivo.PLoS One. 2012,7(7):e40135.(IF=3.24)

43)Cao L, Li Z, Zhang R, Wu Y, Li W,Cao Z*. StCT2, a new antibacterial peptide characterized from the venom of the scorpion Scorpiops tibetanus.Peptides. 2012,36(2):213-220.(IF=3.75)

44)Zhao R, Dai H, Qiu S, Li T, He Y, Ma Y, Chen Z, Wu Y, Li W*,Cao Z*. SdPI, the first functionally characterized Kunitz-type trypsin inhibitor from scorpion venom.PLoS One.2011,6(11):e27548.(IF=3.24)

45)Yan R, Zhao Z, He Y, Wu L, Cai D, Hong W, Wu Y,Cao Z*, Zheng C*, Li W*. A new natural α-helical peptide from the venom of the scorpion Heterometrus petersii kills HCV.Peptides. 2011,32: 11-19.(IF=3.75)

46)Chen Z, Hu Y, Han S, Yin S, He Y, Wu Y,Cao Z*Li W*. ImKTx1, a new Kv1.3 channel blocker with a unique primary structure.J Biochem Mol Toxicol.2011,25(4):244-251.(IF=3.642)

47)Yuan W, Cao L, Ma Y, Mao P, Wang W, Zhao R, Wu Y,Cao Z*, Li W*. Cloning and functional characterization of a new antimicrobial peptide gene StCT1 from the venom of the scorpion Scorpiops tibetanus.Peptides. 2010,31(1):22-26.(IF=3.75)

48)Liu J, Ma Y, Yin S, Zhao R, Fan S, Hu Y, Wu Y,Cao Z*, Li W*. Molecular cloning and functional identification of a new K(+) channel blocker, LmKTx10, from the scorpionLychas mucronatus.Peptides. 2009,30: 675-680.(IF=3.75)

49)Ma Y, Zhao R, Li S, Fan S, Wu Y, Liu H,Cao Z*, Li W*. Characterization of LmTxLP11 and LmVP1.1 transcripts and genomic organizations: Alternative splicing contributing to the diversity of scorpion venom peptides.Toxicon. 2009,53:129-134.(IF=3.033)

50)Yin S, Yi H, Ma Y, Chen Z, Han S, Wu Y,Cao Z*, Li W*. Characterization of a new Kv1.3 channel-specific blocker J123 from scorpionButhus martensii Karsch.Peptides. 2008,29(9):1514-1520.(IF=3.75)

51)Wu W, Yin S, Ma Y, Wu YL, Zhao R, Gan G, Ding J,Cao Z*, Li W*. Molecular cloning and electrophysiological studies on the first K(+) channel toxin (LmKTx8) derived from scorpion Lychas mucronatus.Peptides. 2007,28: 2306-2312.(IF=3.75)

52)Cao Z^#, Xie Y^#, Dai C, Zhu S, Yin S, Wu Y, Li W*. Cloning and characterization of a novel calcium channel toxin-like gene BmCa1 from Chinese scorpion Mesobuthus martensii Karsch.Peptides. 2006,27(6): 1235-1240.(IF=3.75)

53)Cao Z^#, Luo F, Wu Y, Mao X, Li W*. Genetic Mechanisms of Scorpion Venom Peptides Diversification.Toxicon.2006,47: 348-355.(IF=3.033)

54)Cao Z, Dai C, Jiang D, Li W*. The effect of intron location on the splicing of BmKK2 in 293T cells.J Biochem Mol Toxicol.2006,20: 127-132.(IF=3.642)

55)Cao Z, Dai C, Yin S, Wu Y, Sheng J, Sha Y, Li W. Splicing of scorpion toxin gene BmKK2 in HEK 293T cells.J Biochem Mol Toxicol.2006,20: 1-6.(IF=3.642)

56)Cao Z, Mao X, Xu X, Sheng J, Dai C, Wu Y, Luo F, Sha Y, Jiang D, Li W*. Adaptive evolution after gene duplication in α-KTx14 subfamily from Buthus martensii Karsch.IUBMB life. 2005,57: 513-521.(IF=3.885)

57)Cao Z, Xiao F, Peng F, Jiang D, Mao X, Liu H, Li W*, Hu D, Wang T. Expression, purification and functional characterization of a recombinant scorpion venom peptide BmTXKβ.Peptides. 2003,24: 187-192.(IF=3.75)

58)Cao Z, Wu Y, Sheng J, Liu W, Xiao F, Mao Xin, Liu H, Jiang D, Li W. Evidence for the existence of a common ancestor of scorpion toxins affecting ion channels.J Biochem Mol Toxicol.2003,17: 235-238.(IF=3.642)

59)Cao Z, Zhu S, Li W*, Jiang D, He X, Liu H.. Genomic organization of BmTXKβ and BmTXKS2, two scorpion venom peptides from Buthus martensii Karsch.IUBMB life. 2001 ,51: 305-308.(IF=3.885)

60)Li S, Ma Y, Jang S, Wu Y, Liu H,Cao Z*, Li W*. A HindIII BAC library construction of Mesobuthus martensii Karsch (Scorpiones:Buthidae): an important genetic resource for comparative genomics and phylogenetic analysis.Genes Genet Syst.2009,84(6):417-424.(IF=1.517)